This study was a comparative analysis of the antimicrobial and antioxidant effects of Citrus junos, Jeju citrus and extraction of Poncirus trifoliata. The total polyphenols, flavonoids content was the highest in a hot water extraction of Citrus junos while Poncirus trifoliata and Jeju citrus had the highest content of ethanol extracts. The scavenging ability of DPPH radicals and ABTS increased from all extracts depending on concentration. From the result of the antimicrobial experiment, Poncirus trifoliata extracts only have antibacterial effect in S.. aureus. S. epidermidis was observed only in the maximum concentration of the extraction of Jeju citrus. The extracts of Jeju citrus peels have the high antibacterial effect from hot water extraction and ethanol extracts. Based on the results of this experiment, we can know that all the extracts of Citrus junos, Jeju citrus and Poncirus trifoliata can be used as natural antioxidants. Poncirus trifoliata in particular has a remarkable antibacterial effect. Therefore, it is judged that it is possible to use it as a base material for beauty products and cosmetic materials by utilizing these effects.