In this study, chitosan beads combined with Corni fructus (Cornus officinalis) seeds were used as an antifungal agent for phytopathogens. Antifungal activity was measured on R. solani, F. solani, F. oxysporum, and S. sclerotiorum with four different treatments: Corni fructus seeds (CFS), chitosan (CTS), Corni fructus seeds + chitosan (CFS + CTS), and acetic acid (AA). CFS + CTS showed the highest activity based on the EC50 (1.23%) and MIC90 (2.22%) for fungicidal activity of R. solani, while CTS resulted in higher values of EC50 (1.42%) and MIC90 (6.26%). Treatment of CFS + CTS was the highest efficiency value of 51.19 at fungicidal activity of F. solani. The inhibition rate of chitosan beads at pH 3.8 was 100% and 43.5% in R. solani and S. sclerotiorum, respectively. 83% of Kale seedlings were infected at 4 days and 100% at 5 days after treatment of R. solani. Kale seedlings were slightly infected at 3 days, rising to 50% at 5 days after treatment with chitosan beads and R. solani. These results indicate that this bioorganic substance has a great potential as an alternative to synthetic fungicides for the control of plant pathogens.