The objectives of this study were to investigate the effects of trehalose as a cryoprotectant for porcine freeze-driedspermatozoa, to find the optimal freeze-drying time and storage periods of freeze-dried spermatozoa, and to find outpronuclear formation rates, cleaved rates, and embryo development through intracytoplasmic injection of freeze-driedspermatozoa on porcine oocytes. The survival rates of spermatozoa after freeze-drying with trehalose treatment weresignificantly higher than those of them without trehalose treatment (p<0.05). The highest survival rates were foundat 75 mM trehalose treatment. The longer storage periods after freeze-drying seemed to have a lower survival rates. Development in culture of pig by ICSI with trehalose treatment were significantly higher than those of them withouttrehalose treatment (p<0.05). Shorter freeze-drying time of spermatozoa was resulted in the highest cleaved rates andembryo development.