The pyrethrin extracted from Tanacetum cinerariaefolium accounts for two-thirds of the global plant-based insecticide market and is registered as a material that can be used in all countries worldwide. However, due to insufficient research on domestic cultivation techniques for T. cinerariaefolium, a study was conducted to establish cultivation methods by investigating the growth (plant height, width) and flower characteristics (flower weight) and flower yield (number of flowers per plant) of T. cinerariaefolium varieties (0721 strain and Dalmatian) under different planting densities (30×30 cm, 30×40 cm, 30×50 cm, 40×40 cm). The plant height of Dalmatian T. cinerariaefolium was significantly greater than that of the 0721 strain at planting densities of 30×30 cm and 30×40 cm, compared to 30×50 cm and 40×40 cm. The width was also significantly greater for Dalmatian T. cinerariaefolium at all planting densities, except for 40×40 cm, than for the 0721 strain. There were no significant differences in flower yield across different planting densities. The pyrethrin content was significantly higher in Dalmatian T. cinerariaefolium, at 640 ppm/100 g, compared to the 0721 strain. Based on the results of this study, there was no significant difference in flower yield between the 0721 strain...