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논문 리스트

2023
이란 발효 유제품에서 분리한 유산균의 특성
한국낙농식품응용생물학회
오세종 외 1명
논문정보
Publisher
Journal of Dairy Science and Biotechnology
Issue Date
2023-12-31
Keywords
-
Citation
-
Source
-
Journal Title
-
Volume
41
Number
4
Start Page
211
End Page
218
DOI
https://doi.org/10.22424/jdsb.2023.41.4.211
ISSN
27334554
Abstract
This study aimed to identify lactic acid bacteria isolated from eight fermented milk products in Iran. We enumerated Lactobacillus species using De Man-Rogosa-Sharpe (MRS)-maltose and MRS agar with pH adjusted to 5.2, as well as assessment at 37℃ for 48 hr, studied Streptococcus spp. using M17 agar at 43℃ for 24 hr, and assessed Bifidobacterium species using nalidixic acid, paromomycin sulfate, neomycin sulfate, and lithium chloride (BL-NPNL) agar at 37℃ for 48 hr. The total viable Streptococcus spp. cell in fermented milk varied at 4.73?8.83 log CFU/mL. However, Bifidobacterium spp. were not detected in any of the tested samples. Lactobacilli were not detected in four of the eight samples, and viable Lactobacilli cells in the remaining four samples ranged 2.48?3.85 log CFU/mL. The pH of the tested samples ranged 3.53?4.19, and soluble solids (Brix measure- ment) ranged 7.5%?17.9%. A total of 130 isolates of gram-positive catalase-positive bacteria were characterized at the species level using 16S rRNA sequencing. Sequence analysis identified six species: Streptococcus thermophilus, Lactobacillus delbrueckii subsp. sunkii, Lactobacillus delbrueckii subsp. indicus, Lactiplantibacillus plantarum, Lacticaseibacillus rhamnosus, and Levilactobacillus brevis

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