Levilactobacillus brevis NS2301G3, a strain isolated from fermented foods, was analyzed for its complete genome sequenceto identify genes related to probiotic characteristics and functional metabolic pathways. Genome annotation revealed 2,789 protein-coding sequences, highlighting genes involved in carbohydrate metabolism, stress tolerance, and vitamin biosynthesis. Genes responsible for lactic acid production, antimicrobial peptide synthesis, and gamma-aminobutyric acid (GABA) synthesis were identified, emphasizing the potential applications of this strain in fermented food production and probiotic use. CRISPR-associated proteins, exopolysaccharide production genes, and stress response mechanisms further support its adaptability and beneficial characteristics, suggesting its potential as a valuable microbe for human health and food industry applications.