This paper is a study to improve the energy harvesting output of a TENG(Triboelectric nanogenerator) driven by wind power using fine PTFE(Polytetrafluoroethylene) flakes. The structure of the nanogenerator was manufactured in the cylindrical structure, Al(Aluminium) was attached to the inner wall of the cylinder and the PTFE flakes were rotated by the wind inside the cylinder. The number of contact and separation motions was increased as there are multiple PTFE flakes, resulting in improvement of the harvesting output. Through this, it was evaluated to the energy harvesting output characteristics according to the change in the number of PTFE flakes. Up to the optimum, the energy collection efficiency shows the linear correlation with the increase in PTFE flakes and decreases after that. As the PTFE flakes are more than the optimum, the lowering in the harvesting output is induced by obstructing the flow of wind inside the cylinder.