Because bacterial isolates from only a few genera have been developed commercially as biopesticides, discoveryand characterization of novel bacterial strains will be a key to market expansion. Our previous screen usingplant bioassays identified 24 novel biocontrol isolates representing 12 different genera. In this study, we characterizedthe 3 isolates showing the best biocontrol activities. The isolates were Pantoea dispersa WCU35, Proteusmyxofaciens WCU244, and Exiguobacterium acetylicum WCU292 based on 16S rRNA sequence analysis. Theisolates showed differential production of extracellular enzymes, antimicrobial activity against various fungal orbacterial plant pathogens, and induced systemic resistance activity against tomato gray mold disease causedby Botrytis cinerea. E. acetylicum WCU292 lacked strong in vitro antimicrobial activity against plant pathogens,but induced systemic resistance against tomato gray mold disease. These results confirm that the trait ofbiological control is found in a wide variety of bacterial genera.