Objectives: To evaluate the anti-inflammatory and cytotoxic activities of bamboo salt. Methods: Cytotoxicity of bamboo salt and bay salt (0.01%, 0.1%, and 1%) was evaluated using MTT assay. In addition, secretion of the pro-inflammatory cytokines interleukin (IL)-1β and IL-6 from human gingival fibroblasts (HGFs) was measured after application of 0.01% and 0.1% concentrations by using real-time polymerase chain reaction. Results: Bamboo salt and bay salt at 1% concentration were cytotoxic to HGFs at 24 h; however, no such effect was observed at 0.01% or 0.1%. Bamboo salt showed a relatively low inhibitory effect. IL-1β secretion was inhibited by a 0.1% solution of bamboo salt. IL-6 secretion was inhibited by both bamboo salt and bay salt at 0.1% concentration. Conclusions: The above results suggest that bamboo salt inhibits the release of IL-1β and IL-6 from HGFs. Thus, bamboo salt may be a useful material for gingival inflammation. Key Words: Bamboo salt, Human gingival fibroblast, IL-1β, IL-6