This experiment was carried out to investigate the changes of fungal community on fallen leaves of pear by treatment of lime sulfur. Although the lime sulfur could reduce the primary inoculum of several pathogens on spring season, the effect of lime sulfur has not been revealed well scientifically. The fallen leaves infected by pear diseases at pear orchards in Naju were collected and treated with lime sulfur or water as a control. To identify the fungal diversity from each treatment, we analyzed rDNA internal transcribed spacer(ITS) regions after extraction of fungal genomic DNA from lime sulfur-treated or water-treated fallen leaves, respectively. The most common fungal species were turned out to be Ascomycota and Basidiomycota in both treated leaves. However, the population dynamics of several fungal species including Alternari sp., Cladosporium sp., and Phomopsis sp., which are known as pear pathogens for skin sooty dapple disease, were a quite different from each treated leaves. These results indicated that lime sulfur treatment led to changes of fungal communities on pear fallen leaves and could be applicable for a dormant spray.