Purpose: The purpose of this study was to identify the factors affected with nurses'' prevention activity against hospital fall. Methods: The data were collected from 325 nurses at C University Hospital in G City by using the structured questionnaires from February 21, 2011 to March 12, 2011. The data were analyzed by stepwise multiple regression. Results: The main factor associated with prevention activity against hospital fall was the attitude towards hospital fall (β=.26, p<.001), the next one was the educational level (β=.16, p=.002), and the last one was the frequency of fall prevention education (β=.14, p=.009). The all factors could explain 11.1% of the variance in the nurses'' prevention activities against hospital fall. Conclusion: Hospital managers need to make hospital culture to enhance the nurse''s positive attitude about hospital fall prevention. In addition, educators need to develop educational programs including hospital fall prevention through academic curriculum and continuing education.