Licochalcone E is a retrochalcone isolated from the roots of Glycyrrhiza inflate. We previously reported that licochalconeE inhibited IL-12p40 production and attenuated chronic allergic contact dermatitis (ACD). Among the derivativesof licochalcone E, compound #74 ((E)-3-(5-Bromo-4-hydroxy-2-methoxyphenyl)-1-(4-ethoxyphenyl)prop-2-en-1-one) inhibitedthe production of IL-12p40, TNF-α, and IL-6, and NF-κB signaling pathway from LPS stimulated RAW264.7 macrophages. The effect of compound #74 (0.5, 1, and 5%) on ACD model was very weak.