This study evaluated the mixed light-emitting diode (LED) module for dental care, which promotes cell regeneration. To evaluate the mixed LED module (634 nm + 860 nm), human gingival fibroblast (HGF-1) was irradiated on the mixed LED light at 0 and 12 h after cell scratch. The wound area was observed in 0, 6, 12, 24, and 48 h. As a result, the mixed LED light irradiation affected wound healing. The highest wound area reduction rate was confirmed when the mixed wavelength of 634 nm + 860 nm was irradiated for 400 s. In this study, the mixed LED light irradiation only varied in irradiation time. However, each group showed a different wound area reduction rate. In conclusion, this study confirmed that the fastest wound healing effect occurred when irradiation was employed with mixed LED for 400 seconds on human gingival fibroblast. Through the study results, light irradiation time should be considered for the mixed LED light irradiation wound healing effect. In order to more accurately verify the wound healing effect of the mixed LED light irradiation, it will be necessary to compare each wavelength of light (634 nm or 860 nm) using HGF-1 cells.