This study was carried out to apply RAPD marker technology for a half-sib breeding strategy. Thirty two rose cultivars comprising eight parents and twenty four F1 progenies were used to determine parentages. Dendrogram based on cluster analysis consisted of four groups. In total 188 bands were scored from 20 primers. We were able to determine parentage of F1 progenies by a cluster and principal coordinate analysis. The RAPD marker can be used as a useful tool for parentage identification. Consequently, the technique of parentage determination using a RAPD marker will be useful in half-sib breeding strategy of roses.