The fungal endophyte EML-CM25 was isolated in Korea from surface sterilized Chinese maple leaf tissues. Internaltranscribed spacer sequence analysis indicated that the isolate showed 99% sequence similarity with Paraconiothyrium brasiliense,a finding that was confirmed by morphological analyses. The fungal colonies did not express aerial hyphae. Conidiomataformation was observed in the fungus cultured on potato dextrose agar at 25oC for 7 days. Visible scattered black dots consistingof pycnidia were present throughout the colony. This is the first record of P. brasiliense in Korea.