A previously unrecorded fungus was isolated from the persimmon (Diospyros kaki) leaf phyllosphere in Korea. The isolated fungus was characterized by morphological and phylogenetic analyses. The typical morphological characteristics of Phialocephala piceae, including dark brown colonies and short, thick conidiophores, were observed on the isolated fungus. A phylogenetic analysis based on the internal transcribed spacer (ITS) region and RNA polymerase II largest subunit (RPB1) also confirmed the identification of the isolated fungal species as P. piceae. Therefore, this is the first report of P. piceae in Korea.