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논문 리스트

2010
피조개, Scapharca broughtonii (Schrenck) 정자의 냉장보존에 미치는 희석액의 효과 Effect of Diluents on the Cold Storage of Sperm in Scapharca broughtonii (Schrenck)
한국패류학회
고강희
논문정보
Publisher
The Korean Journal of Malacology
Issue Date
2010-06-01
Keywords
-
Citation
-
Source
-
Journal Title
-
Volume
26
Number
2
Start Page
145
End Page
149
DOI
ISSN
12253480
Abstract
The effects of diluents composition on cold storage for Scapharca broughtonii (Schrenck) sperm were examined in the percentage of sperm activity and survival rate. Various diluents of glucose solutions (10 mM Hepes-pH 7.8), 600 mM NaCl, stein solution, Ringer’s solution (230 mM NaCl, 8 mM KCl, 2 mM CaCl2, 3.7 mM MgCl2, 0.2 mM NaHCO3, 10 mM Hepes-pH 7.8), 20%, 25% ASW (NaCl 2.7 g + KCl 0.07 g + CaCl2 0.12 g + MgCl2 0.46 g + NaHCO3 0.05 g + distilled water 100 ml) were used to store th sperm at 4℃. The storage effect was evaluated using sperm activity and survival rate. Ringer''s solution was found to be better diluents which maintained high activity and survival rate of sperm for a storage period of 7 days. Optimal pH of diluents to store the sperm at 4℃ is 7.5.

저자 정보

이름 소속
고강희 양식생물학과