In this study, a comparative gene expression based on acute thermal stress (34 °C) exposure in Pacific abalone was conducted using transcriptome analysis. The hemocyte tissue were collected at 3 h (HS group) and 8 h (HT group) to conduct transcriptome analysis. In RNA sequencing, 132,199,030 transcriptome short reads were generated, and the average length was 125.59 bp. Among the expressed 9,721 genes, 8,302 were identified as functional. In DEG selection, 195 up-regulated genes and 512 down-regulated genes were selected, wherein, 168 up-regulated and 456 down-regulated genes were identified in the functional description. Within up-regulated DEGs, 11.2% were involved with biological process, 15% with cellular component, and 52.5 % with molecular function while within down-regulated DEGs, it was 20.2%, 21.8%, and 22.6%, respectively. The expression values were compared by measuring the average read count of heat shock proteins in each of the two experimental groups. HSP90 was expressed in 64,594 in the HS group and 22,649 in the HT group, following HSP60, 420 in the HS group and 94 in the HT group, HSP20, 87 in the HS group and 27 in the HT group. These results suggest that acute thermal stress alters the body mechanism of Pacific abalone.