Chironomid larvae in drinking water treatment plants (DWTPs) and tap water lead to highlights the urgent need for effective control measures. The use of combined disinfectant treatments is becoming increasingly common to achieve optimal disinfection efficiency while minimizing residual contamination. This study was conducted to study the effect of combined treatment of disinfectants used in DWTPs on the biological control potential of chironomid larvae. In the experiment, Glytotendipes tokunagai larvae reared according to OECD guidelines were exposed to NaOCl, UV, and O3 complex substances, and biological responses were analyzed using survival rate and body color change as key indicators for 24 hours. The survival rate of larvae exposed to combined disinfectants mostly decreased in a time- and concentration-dependent manner, and lightening of body color was observed. The most significant decrease (53%) of the survival rate was observed to a combination of UV and 4 ppm NaOCl disinfectants and body color change also showed a statistically significant difference compared to the control group (P<0.001). The change in body color is suggested to be a physiological change caused by oxidation of hemoglobin due to exposure to disinfectant complexes. The results of this study contribute...