In this study, we studied the use of 10 commercial disinfectant candidates in aquaculture field conditions for fish pathogenic viruses; Marine birnavirus, Infectious pancreatic necrosis virus, Hirame rhabdovirus. Sodium hypochlorite inactivated more than 99% under the treatment conditions of 23 ppm for 20 minutes, 150 ppm for chlorine dioxide, 40-80 ppm for povidone-iodine, 50 ppm for glutaraldehyde, and 150 ppm for didecyl dimethyl ammonium chloride. The virus inactivation ratio exceeded 99% under the treatment conditions of hydrogen peroxide at 1,050 ppm for 60 minutes and Formalin at 500 ppm or more, and the virus inactivation concentration of hydrogen peroxide and formalin was too high. These indicate that its disinfection effect was insignificant. Quaternary ammonium compounds were not expected to inactivate viruses even at 225 ppm of active ingredient, dichlorobenzen at 2,250 ppm, and copper sulfate at 3,000 ppm, so it was showed that it could not be used as a disinfectant. As a result of this study, sodium hypochlorite, chlorine dioxide, povidone-iodine, glutaraldehyde, and didecyl dimethyl ammonium chloride were confirmed to have antiviral effects.