The aim of this study is to find out the behavior and acoustic backscattering of the large jellyfish Nemopliema nomurai using hydroacoustics in situ. N. nomurai was distributed at depths ranging from 10~15 m during the day. Regarding the behavior of N. nomurai, there was no significant change in depth, and 3D tortuosity was not high. The vertical direction was ±10° from the horizontal, and moving speed was 0.9~1.5 m s?1.With regard to hydro?acoustical characteristics, the mean TS of N. nomurai ranged from ?69.6~?56.0 dB at 38 kHz and ?69.4~?54.5 dB at 120 kHz. TS variation (Max TS?Min TS) at 38 and 120 kHz was 0~10.2 dB and 0.2~16.0 dB, respectively. Mean TS and TS variation (Max TS?Min TS) of N. nomurai were higher at 120 kHz than at 38 kHz. The results showed that the use of hydroacoustics was effective in estimating the distribution depth, behavior, and acoustic characteristics of the target.