Background: Heat stress during summer impairs reproductive performance in sows, causing summer infertility. Cytochrome P450 1A2 (CYP1A2) plays a crucial role in steroid hormone metabolism in the liver and has been shown to be upregulated under stress conditions. However, the effect of seasonal heat stress on CYP1A2 expression in sow ovaries remains poorly understood. Methods: Ovaries were collected from crossbred sows during different seasons in Korea. The control group (CON) was exposed to optimal temperatures for swine rearing (22.0-24.5℃) during spring, while the heat-stressed group (HS) experienced high temperatures (30.5-37.0℃) during summer. Temperature-humidity index (THI) was calculated to assess thermal stress severity. CYP1A2 expression was analyzed using Western blotting and immunohistochemistry (IHC), and these values were statistically compared with THI by Pearson’s correlation analysis. Results: The HS exhibited significantly higher THI values compared to the CON (88.11 vs. 66.67). Western blot analysis revealed significant upregulation of CYP1A2 expression in the HS compared to CON (1.89 vs. 1.00). IHC demonstrated that CYP1A2 was specifically localized in granulosa cells of mature follicles, with a significantly higher proportion of CYP1A2-positive follicles in ...