The fishing industry has a negative effect on the environment due to greenhouse gas (GHG) emissions with the high use of fossil fuels, the destruction of underwater ecosystems by bottom trawls, reduction in resources by fishing, and altered ecosystem diversity. GHG emissions from fisheries were discussed at the Canc?n meeting in Mexico in 1992 and are part of the Kyoto protocol in 2005. However, few studies have investigated the GHG emissions from Korean fisheries. To find a way to reduce GHG emissions from fisheries, quantitative analysis of GHG emissions from the Korean fishery industry is needed. Therefore, this study investigated the GHG emissions from the Korean Danish seine fishery using the life cycle assessment (LCA) method. The system boundary and input parameters for each process level are defined for the LCA analysis. The fuel-use coefficient of the fishery is also calculated. The GHG emissions from the representative fish caught by the Danish seine fishery are considered and the GHG emissions for the edible weight of fishes are calculated, considering consumption in different areas and different slaughtering processes. The results will help to understand the GHG emissions from Korean fisheries.