In this study, phytochemicals extracted from sea mustard (SM) and sea mustard sporophyll (SMS) in ethanol solution have been analyzed and wound healing potential of the phytochemicals was investigated. In the phytochemical screening studies, the extract of SM and SMS includes several phytochemical compounds such as phytol, ascorbic acid, sitgmasta, fucosterol and ergosta. Cytotoxicity studies of the extract of SM and SMS with mouse macrophage RAW 264.7 cells showed on toxicity up to a high concentration of 1.0 mg/mL. Furthermore, the SM and SMS extract significantly reduces the production of nitric oxide (NO) induced lipopolysaccharide on RAW 264.7 cells with a dose-dependent manner. In addition, the extract of SM and SMS has the effect of enhancing the cell migration and invasion of fibroblast. These results demonstrate that the extract of SM and SMS could help to heal wound by reducing NO production and increasing cell migration