Xanthophyll carotenoids are pigments composed of terpene structures found in various organisms. They have attracted significant attention in the food, nutrition, pharmaceutical, and cosmetic industries due to their remarkable antibacterial, anti-inflammatory, and antioxidant activities. Among these, Deinoxanthin, produced by microorganisms of the Deinococcus genus, is known for its superior radical scavenging activity compared to well-known carotenoids like lutein, beta-carotene, and zeaxanthin, owing to its unique structural specificity. The aim of this study was to optimize carbon sources to establish efficient production platform for deinoxanthin using Deinococcus radiodurans. Glycerol proved to be the most effective carbon source for the synthesis of deinoxanthin among those that were examined. From optical density at 600 nm of 37.5 of D. radiodurans, more than 19.8 mg/L of deinoxanthin was produced. Furthermore, the viability of employing inexpensive carbon sources was tested using crude glycerol. A productivity of 20.2 mg/L of deinoxanthin was produced from the optical density at 600 nm of 37.5 of the cell after 72 hours of fermentation. These findings indicated that the D. radiodurans strain might be used to produce carotenoids in a cost-effective manner using cheap ...