Pumpkin has long been used as traditional health materials in oriental medicine, pharmacy, medicine, and food industries on many countries. In this study, the water extract and two active components from tendril of young C. moschata Duch. were investigated on inflammation inhibitory activity. The water extract (0.313 mg/mL - 2.5 mg/mL) did not give any affect the cell viability over 95% and lipo-polysaccharide (LPS) as positive control co-treated Bone Marrow-Derived Macrophages depend on the concentration of water extract of young C. moschata Duch. dramatically decreased the secretion level of inflammatory cytokines such as an interleukin 6 (IL-6) and a tumor necrosis factor α (TNF­α). Also, two active fractions (B4 and D3, 0.1 mg/mL - 1.0 mg/mL) isolated from water extract of young C. moschata Duch. showed to be strongly suppressed IL-6 and TNF­α. Among the domestically cultivated pumpkins, two active fractions comprised the tendril of the young and old of C. moschata Duch. and Cucurbita pepo var. cylindrica. These results suggest that water extracts of C. moschata Duch. and its active components show a potential anti-inflammation activity by decrease of the secretion of IL-6 and TNF­α. It can be applicable for pharmaceutical materials.