This study conducted a survey using a sampling inspection method and acoustic technique for continuous monitoring of useful organisms and non-commercial fish species distributed in the water of Gwangyang Bay, and evaluated the marine resources. In June, 2018, 38 species were caught in the shrimp beam trawl, comprising 3,594 marine organisms with a total weight was 65,415.3g. The acoustic survey showed that the nautical area scattering coefficient (NASC; m2/n.mile2) for the waters of Gwangyang Bay was highest in Transect 15 (1,655.5 m2/n.mile2) and lowest in Transect 1 (1.3 m2/n.mile2). The biomass was estimated to be 2,634.9 tons in the acoustic survey. This indicates that the acoustic survey method detected more fish shoals than the sampling inspection method.