This study is a study to empirically verify the mediating effect on college life adaptation and career preparation behavior of college students. The purpose of this study is to empirically analyze the multi-mediated effects of major satisfaction, job stress, and self-directed learning. For this study, 216 university students were enrolled. For the collected data, SPSS Process macro was used. The result is as follows. First, there were multiple parallel mediating effects and multiple serial mediating effects on major satisfaction, job stress, and self-directed learning between college life adaptability and career preparation behavior. Second, the path of simple mediation and double mediation effect was found between college life adaptation and career preparation behavior. Based on the research, the necessity of revitalizing the program for revitalization of teaching activities and industry-academic cooperation activities in the major field and improvement of career preparation behavior and university life adaptation ability and follow-up research were suggested.