In this study, we researched the characteristics of germs that produce a bioflocculant, which is harmless and has a high flocculating activity used for the purpose of application to water treatment and other industrial processes that could replace chemical flocculants currently in use, through isolation and identification. Also, in order to increase the flocculant output and flocculation efficiency, we investigated the optimized culture conditions and environments such as flocculant optimal dose, pH condition, temperature condition, and et cetera. As a result, by conducting separation of the flocculant producing new microorganisms and identification through 16S rRNA sequencing, it exhibited 97% homology with Delftia acidovorans strain NBRC 14950, and thus we named it strain YG-02. Strain YG-02 grew most optimally in pH 7.0 range and 22oC and the maximum flocculating rate of strain YG-02 that grew in the optimal condition turned out to be 92.73%. As a result of this study, we discovered a new microorganism that produces a bioflocculant and a possibility of replacing chemical flocculants.