The Antarctic krill Euphausia superba is important commercially and ecologically as a basic component of the Antarctic Ocean ecosystem. To manage this resource, it is important to determine the distribution and standing of krill in the water layer. Acoustic methods can capture information about the entire water layer quickly. Acoustic surveys were conducted from March 3 to March 14, 2017, using the commercial fishing boat Sejong (7,765 tons). Acoustic systems with a frequency of 38 kHz and a 200 kHz commercial echo sounder (ES70, Simrad, Norway) were used and the acquired data were processed using post processing software. The density and standing of Antarctic krill were determined using the two-frequency difference method, using the characteristics of two frequencies. To compare the frequency difference of krill, the method using the frequency difference according to the krill length, recommended by the Commission for the Conservation of Antarctic Marine Living Resources (CCAMLR) and the values extracted according to the krill length at survey stations where only krill were collected during the study period, were compared. The frequency difference ranges were 3.96-5.91 dB and -3.0~13.8 dB, respectively.