In this study, we investigated whether it is possible to substitute the inorganic zinc of chitosan oligosaccharide substrate organic zinc. We investigated the quality characteristics of egg produced from laying hens with non-supplemented diets or diets supplemented with organic zinc on chitosan oligosaccharide. Organic-zinc was obtained from substrate of chitosan oligosaccharide. Organic zinc or non-organic zinc were mixed normal laying hen diet. Laying hens were fed diet for 45 days containing powdered chitosan oligosaccharide-Zn, inorganic zinc or normal feedstuff. During the experimental period, organic zinc group increased the egg shell thickness, egg heights and lymphocyte compared to inorganic zinc or aminated form products (p<0.05). And, edible weight and yolk colors were no significant difference. But, each effectiveness are different from organic-zinc concentration to feeding period. It was investigated that organic zinc had equal or more effects compared with inorganic zinc or normal diet. These results suggest that organic zinc could be used as substitute for inorganic zinc as well as the supplement of laying hens diets to produce functional eggs.