This study aims to develop ecofriendly textile products that exhibit antimicrobial properties. The surface of the bamboo/cotton blended fabric was treated with chitosan to improve the fabric’s accessibility to natural acidic colorant, and to imbue it with chitosan’s antimicrobial properties. The effect of the chitosan treatment was evaluated in terms of the following properties of the fabric after dyeing it with natural pomegranate-rind extract: dye uptake, color, colorfastness, antimicrobial activity, and physical properties like stiffness, wettability, and air permeability. For the fabrics treated with 0.5% chitosan concentration, the color yield (K/S value) improved by 2?3 times (depending on the colorants’ concentration) compared to that of the untreated fabric. The shade of dyed fabrics was not changed by the chitosan treatment, and the fabrics continued to have the same Y Munsell color series as before. Colorfastness was not affected by the chitosan treatment either. However, with chitosan treatment, the fabrics got stiffer, more air permeable, and more water repellent. The treated fabrics also showed excellent antimicrobial activity against S. aureus and K. pneumoniae.