A 10-year-old spayed female Yorkshire Terrier visited for a physical checkup. The bitch had a history of ovariohysterectomy for treatment of pyometra a year ago. On physical examination, the dog was bright and alert. Complete blood counts, serum biochemistry and blood gas analysis results did not show any deviations within normal ranges. Radiographic and ultrasonographic examinations revealed unilateral hydronephrosis and hydroureter of the right kidney and ureter, and obstruction of the distal ureter was observed. On the basis of these results, nephroureterectomy was performed. During the operation, the adhesion of the distal ureter and surrounding tissue cells were observed without the evidence of the ureteral ligation. The distal ureteral obstruction was presumed to be adhesion caused by fibrous tissue formation between ureter and retained broad ligament, or incompletely removed blood clots following ovariohysterectomy. This case report describes the occurrence of hydroureteronephrosis caused by adhesion of the distal ureter following ovariohysterectomy in a bitch.