We investigated the quality characteristics of egg produced from laying hens with non-supplemented diets and diets supplementedwith organic minerals on chitosan oligosaccharide. We are made of organic-minerals on substrate of chitosan oligosaccharide. Organic minerals were mixed normal laying hen diet. Laying hens were fed diet for 45 days containing powdered chitosanoligosaccharide-Ca, Zn, Se or normal food-additives. The egg weights, color of egg components, egg shell thickness, eggweights, and albumen high were increased compared to normal food-additive group (p<0.05). But, each effectiveness are differentfrom organic-minerals. Overall, organic zinc were more effects compared with other organic-calcium and selenium minerals. But, organic selenium was found the decreased body weight and egg weight. These results were happened because of highleveldosage. These results suggest that organic minerals possesses the potential as supplement of laying hens diets to producefunctional eggs.