In this paper, we examine whether the 3D image experience through a light-field display device showed the difference in the arousal of the user compared with the 2D image experience. For our experiment, the Looking GlassTM (LG) was used as a lightfield display device that provided 3D images, and 2D images were provided by digital and printed images. The subject’s facial behavior during each media experience was recorded for analysis and the degree of arousal was measured by FaceReaderTM. As a result, the first image presented in the first order among the three kinds of images showed that there was a statistical difference in the degree of arousal between the three media. However, no significant differences were found between the three media in the other images. This may be because the arousal did not increase from the experience of the second image through the LG, owing to habituation. In conclusion, the 3D imaging experience may appear in the beginning, but does not continue.