Hydroacoustic surveys were conducted for spatio-temporal distribution and size estimation of fish in the Yongdam reservoir, Korea, from April to July 2014. Acoustic data were collected along acoustic transects using a 120 kHz scientific echosounder. The received acoustic data were the in situ acoustic target strength (dB) and nautical area scattering coefficient (m2/mile2). Data on fish behavioral patterns and size were collected using a DIDSON acoustic camera at stationary stations. Fish were mainly distributed in the center channel and close to the outer Yongdam reservoir. Acoustic density of fish in the summer season were higher than in the spring season. The seasonal vertical distribution pattern of fish aggregations may be strongly related to the vertical temperature structure. The size distribution of fish obtained from an acoustic camera correlated well with the acoustic size of fish from an echosounder.