The purpose of this study was to investigate the utility of a bamboo-distilled extract as a cosmetics ingredient by studying the extract’s antimicrobial, antifungal and antioxidative properties. Results indicated that when the cell viability was less than 3.0% in the MTT assay of Normal Human Dermal Fibroblasts and Normal Human Epidermal Keratinocytes, there was no toxicity, suggesting that the extract met established safety standards. The antimicrobial activity against Staphylococcus aureus, Escherichia coli and Salmonella typhymurium was 0.625% at a minimum inhibitory concentration. Staphylococcus aureus had a minimum lethal concentration of 2.5%, and Escherichia coli and Salmonella typhymurium had a minimum lethal concentration of 5%. At a concentration of 25%, the clear zone of M. Canis was 10mm, and T. rubrum, T. Schoenleinii and M. ferrugineum had a clear zone of 7mm. At a concentration of 50%, M. Canis, T. Rubrum, T. Schoenleinii and M. Ferrugineum had a clear zone of 16mm. The DPPH free radical scavenging activity was 3%, indicating the extract had an antioxidant effect. These findings suggest that the tested bamboo-distilled extract is applicable as a natural material in beauty products based on its high level of antimicrobial, antifungal and antioxidative activities.