This paper presents an endosymbiotic evolutionary algorithm (EEA) to solve both problems of line balancing and model sequencing in a mixed-model two-sided assembly line (MMtAL) simultaneously. It is important to have a proper balancing and model sequencing for an efficient operation of MMtAL. EEA imitates the natural evolution process of endosymbionts, which is an extension of existing symbiotic evolutionary algorithms. It provides a proper balance between parallel search with the separated individuals representing partial solutions and integrated search with endosymbionts representing entire solutions. The strategy of localized coevolution and the concept of steady-state genetic algorithms are used to improve the search efficiency. The experimental results reveal that EEA is better than two compared symbiotic evolutionary algorithms as well as a traditional genetic algorithm in solution quality.