The aim of this study was to compare physicochemical properties of solid lipid nanoparticles (SLN) made from different lipids. To make small, stable, uniform and highly encapsulated SLNs, many factors such as the components (lipid, stabilizer) and preparation condition (sonication time, power) can be considered. Out of those, we selected solid lipid as lipid matrix to investigate an effect on SLNs. The SLNs were characterized by particle size, zeta potential, solubility and in vitro release study. In this study, SLNs showed different physicochemical properties and release profiles according to used solid lipid. In case of particle size, M-SLN showed biggest particle size (412.5 ± 29.4 nm) and highest encapsulation efficiency (61.2 ± 4.8 %). And, B-SLN showed highest cumulative drug percentage (85.0 ± 1.7 %, 24 h) in release study. These results suggest that lipids type affect physicochemical properties and release profile of SLN.