To determine sulfate uptake and assimilation of various rape cultivars(Brassica napus L.) under different S levels, the activity of ATP sulfurylase, SO42－ uptake and glutathione(GSH) concentrations were measured in different age of leaves. In this study, ten rape cultivars(Mokpo, Tamra, Youngsan, Naehan, Saturnin, Akela, Mosa, Capitol, Pollen, and Colosse) showed various sulfate uptake and assimilation capacity in response to inefficient sulfate supply. Under inadequate sulfate conditions, a greater ATP sulfurylase activity in young leaves was observed in all cultivars compared to that of old leaves. In addition, GSH concentration was considerably increased in young leaves when S supply was declined from 2.0 to 0.2mM. These results suggested that rape cultivars differ in their capacity to utilize sulfate under limited S conditions.