It is desirable that a wound healing membrane acts as a barrier for coverage of a damaged skin and has the biological activities such as anti-inflammatory effects. In this study, we prepared the hydrogel film containing the propolis nanoparticles as a wound healing membrane. The propolis nanoparticles were prepared by incorporation of propolis into the hydrophobic core of γ-cyclodextrin. The incorporation efficiency of propolis in the nanoparticles was 50±2.3%. Propolis nanoparticles observed by a scanning electron microscope (SEM) were spherical with the size of 30~40 nm. The swelling behaviors of the hydrogel film containing propolis nanoparticles showed a similar pattern with the hydrogel film without propolis nanoparticles. The cumulative amount of propolis released from the hydrogel film containing propolis nanoparticles in the buffer of pH 7.4 and 5.5 was 86.0±2.0% and 64.6±1.0% of total propolis loaded in the hydrogel film within 9 h, respectively. These results provide a rationale for studying wound healing application of the hydrogel film containing propolis nanoparticles in a clinical setting.