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2011
Random amplified polymorphic DNA와 fluorescent amplified fragment length polymorphism 분석을 통한 Salmonella spp.의 유전형 분석
Genotyping of Salmonella spp. by random amplified polymorphic DNA and fluorescent amplified fragment length polymorphism analysis
한국수의공중보건학회
논문정보
- Publisher
- 한국수의공중보건학회지
- Issue Date
- 2011-12-23
- Keywords
- -
- Citation
- -
- Source
- -
- Journal Title
- -
- Volume
- 35
- Number
- 4
- Start Page
- 220
- End Page
- 230
- DOI
- ISSN
- 12251739
Abstract
Random amplified polymorphic DNA (RAPD) and fluorescent amplified fragment length polymorphism (FAFLP)
analyses were executed on a total of 28 Salmonella spp., including 6 ATCC reference strains, 2 isolates from outbreaks
of food poisoning in Gwangju, and 20 isolates from carcasses. For RAPD analysis, four primers, named P1254, 23L,
OPA-4, OPB-17 were used producing amplification fragments ranged from 0.18kb to 2.6kb. As a result, 5 types using
P1254, 5 types using 23L, 3 types using OPA-4, and 6 types using OPB-17 and a total of 18 RAPD types were achieved.
For FAFLP analysis, bacterial genomic DNA was digested with endonucleases EcoRⅠ and MseⅠ, site-specific adaptors
were ligated, and PCR amplification was carried out with an EcoR1 adaptor-specific primer labelled with fluorescent
dye. Amplified fragments, which were separated on a polyacrylamide sequencing gel ranged from 35bp to 300bp were
analysed. Results were displayed as a dendrogram with genetic distance. Twenty two Salmonella isolates and 6 reference
strains were divided into 14 groups in a level of 0.136 genetic distance. In conclusion, Salmonella isolates of chicken
carcasses have different genetic properties when compared to reference strains and isolates from outbreak of food
poisoning.
- 전남대학교
- KCI
- 한국수의공중보건학회지
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