Purpose: The purpose of this study was to construct a questionnaire to assess male workers’ sobriety behavior based on Ajzen''s Theory of Planned Behavior (TPB). Methods: A primary questionnaire with 56 questions was constructed based on literature review and structured interview with male workers. The Content Validity Index (CVI) was evaluated by a group of experts, construct validity was tested by principle component analysis, and reliability was evaluated by Cronbach''s？and the test-retest reliability test. Results: Fifty two questions showed higher than .8 of CVI. Four factors explained 78.71% of the total variance among items for sobriety intention and direct measure, and six factors explained 67.99% of the total variance among indirect measure items and those factors coincided with the variables of TPB in factor analysis. Cronbach''s？of this questionnaire was .873 and the result of test-retest reliabilty test was relatively reliable. A total of 41 items with 7-point scale were constructed in the final version. Conclusion: This questionnaire was valid and reliable to measure sobriety behavior based on TPB in male workers. It can be useful to evaluate the result of a sobriety program for male workers.