Salmonella enterica serovars Gallinarum(SG, causative agent of fowl typhoid) and S Pullorum(SP, causative agent of pullorum disease) are very important bacterial pathogens in poultry industry. They share some common antigenic properties though the characteristics of outbreaks are quite different. To discriminate between SG and SP, we developed two rapid diagnostic methods, allele-specific real-time PCR and 3''-tailed PCR over 2 single nucleotide polymorphisms (237th and 598th). In both methods, 237th allele was found to be a good target for differential diagnosis, while 598th allele produced some non-specific reactions.