Natural products made from plant sources have been used in a variety of cosmetic applications as a whitening, wrinkle and inflammatory agent. The Artemisia princeps have been used as a traditional medicine for skin diseases and have been reported to possess anti-oxidant properties. Artemisia princeps was extracted with 100%, 80%, 50% methanol or ethanol and then total flavonoid content (TFC) and total phenolic content (TPC) of their extracts were measured using the sodium borohyride/chloranil-based assay and Folin-Ciocalteu reagent reaction, respectively. DPPH radical scavenging activity of their extracts were measured by 1,1-diphenyl-2-picryl hydrazyl (DPPH) method. Fifty % methanolic extracts among the various extracts showed the highest TFC and TPC, and then 100% methanolic extracts showed the highest DPPH radical scavenging activity. Two flavonoids (eupafolin and eupatilin) were isolated from 100% methanolic extract of A. princeps. The DPPH radical scavenging activity of eupafolin and eupatilin was showed IC50 value of 35.7 μg/mL and 32.6 μg/mL by 1,1-diphenyl-2-picryl hydrazyl (DPPH) method. Therefore, the eupafolin and eupatilin displayed a preventive effect when used for anti-oxidant purposes in human skin and may be an appropriate choice for cosmetic products that aim to provide dermal anti-oxidant effects.