ABSTRACT : This study was performed to compare effect of immune activities of Rhodiola sachalinensis by various extraction process with different temperature and extraction solvents. Experiments were performed for investigate the immune activities on human B and T cell growth and secretion of their cytokines. Also, antibodies in serum were investigated in female ICR mouse by feeding the extracts of R. sachalinensis at doses of 40, 120 and 360 ㎎/㎏ orally for 15 days. The immune cell growth and secretion of cytokines (IL-6, TNF-α) on human B and T cells were increased by adding R. sachalinensis extracts compare to the control. Also, total serum IgG levels increased by feeding R. sachalinensis extracts. It can be conclude that optimum condition for efficient extraction of R. sachalinensis as functional material is slovent extraction process using water with ultrasonification at below 100℃ than typical process. Key Words : Rhodiola sachalinensis, Ultrasonification, Immune Activity, Cytokine, IgG, NK cell