This study aimed to find out the effect of stereotypes of hotel employees on teamwork and whether there is a moderating effect of organizational trust in the relationship between stereotypes and team members. For this study, a survey was conducted targeting hotel employees in Seoul for 40 days from December 5, 2016 to January 13, 2017 and 244 copies of questionnaire were used for empirical analysis. As a result of the analysis, the factors of stereotypes affecting teamwork were personal factors and job factors, and there was a moderating effect of the organizational trust between stereotypes of hotel employees and teamwork. Therefore, various programs should be prepared so that the communication between the hotel workers can be continuously made for better understanding, and hotels should gain the trust of their employees with a consistent goal of the organization and proper management so that teamwork will improve.