ABSTRACT : This study was performed to investigate the increasing of immune activities from Centella asiatica L. Urban by several solvent extraction with ultrasonification process. Ethanol extracts at 60℃ and associated with ultrasofication showed more 15% yield than normal 100℃ water extracts. And ethanol extracts showed higher yield than water extraction at each process. It can be concluded that yield due to polarity of compound in C. asiatica from HPLC peak. All of extracts from C. asiatica revealed lower cytotoxicity than 32%. The ethanol extracts with ultrasofication showed higher promotion on human B and T cell growth, about 10% compared to the control. The secretion of IL-6 and TNF-a was also enhanced as 4.86 × 10？4 pg/cell and 5.73 × 10？4 pg/cell each, by the addition ethanol extracts with ultrasonification to T cell. NK cell activation was improved up to 10% higher than the control, through adding the samples. It can be concluded that the ethanol extracts from C. asiatica has higher immune activities followed by ultrasonification process, possibly, by higher yield than conventional extraction process. Key Words : Centella asiatica L. Urban, Immune Activity, Solvent, Extraction, Ultrasonification