To identify materials possessing adermal bioactive properties, this study was conducted the effects of Opuntia ficusindica fruits fermented with Steptococcus thermophilus (OFST) on cytotoxicity of human dermal fibroblast, antioxidant activity, collagen biosynthesis and matrix metalloproteinase-I (MMP-1) activity. As the results, the number of viable bacteria for OFST after fermentation for 12 h showed 0.4 log CFU/mL and it exhibited 8 log CFU/mL after fermentation for 24 h. The initial pH of OFST was 7.2. The pH between 12-24 h was 6.4 and 4.7, respectively, The pH of OFST after fermentation for 24 h was 4.5. The OFST didn’t show cytotoxicity against human dermal fibroblast at tested concentration. The OFST didn’t show cytotoxicity against human dermal fibroblast at concentration of 1-100 μg/mL. In antioxidant activity, OFST showed significant result of scavenging action over 10 μg/ml. The OFST have significantly antitoxidant activity with dose-dependent manner. In biosynthesis of collagen, OFST represented a meaning effect. It was increased with dosedependent manner over 10 μg/ml. In effect on collagen synthesis, TNF-α treatment was appeared to have a effect on increasing MMP-1 activity compared with TNF-α nontreatment. However, OFST treatment on increased MMP-1 activity was decreased it. The significant effect of OFST on MMP-1 activity was showed