Objectives: The purpose of this research was to identify the relationship between state-trait anxiety, parenting stress and parenting self-efficacy in mothers of children with Amblyopia. Methods: This study was surveyed 221 mothers of children aged 3 to 14 years with amblyopia who have visited ophthalmology of outpatient department of a university hospital and been treated with occlusion therapy in G-city. The collected data were analyzed by t-test, ANOVA, correlation coefficient, using the SPSS program. Results: There was significant positive correlation between state-trait anxiety and parenting stress (r=.480, P<0.001) and negative correlation between state-trait anxiety and parenting self-efficacy (r=-.402, P<0.001). Parenting self-efficacy had negative correlation to parenting stress (r=-.484, P<0.001). Conclusions: Nursing intervention program which enhances the self-efficacy and reduces the parenting stress in mothers of children with amblyopia should be developed to improving vision of the children.